ELECTRON-TRANSPORT CONTROLS TRANSCRIPTION OF THE GLUTAMINE-SYNTHETASE GENE (GLNA) FROM THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6803

被引:45
作者
REYES, JC
FLORENCIO, FJ
机构
[1] UNIV SEVILLA, CSIC, DEPT BIOQUIM VEGETAL & BIOL MOLEC, E-41080 SEVILLE, SPAIN
[2] UNIV SEVILLA, CSIC, INST BIOQUIM VEGETAL & FOTOSINTESIS, E-41080 SEVILLE, SPAIN
关键词
CYANOBACTERIA; GLNA GENE; GLUTAMINE SYNTHETASE; SYNECHOCYSTIS SP PCC 6803; LIGHT REGULATION; NITROGEN ASSIMILATION;
D O I
10.1007/BF00020231
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The glnA gene, encoding type I glutamine synthetase (GS) in Synechocystis sp. PCC 6803, showed a high sequence similarity with other cyanobacterial glnA genes. A dramatic decrease in the amount of glnA mRNA, a single transcript of about 1.6 kb, was observed after transfer to darkness, or after incubation with the electron transport inhibitors DCMU or DBMIB. The levels of glnA transcript were fully recovered after 5 min of reillumination. The glnA mRNA was found to be equally stable both in the light and the dark (half-life about 2.5 min). Unlike the glnA messenger, the amount of GS protein was not reduced in the dark. Synthesis of the glnA transcript in the dark required the presence of glucose. In addition, glnA transcription in a Synechocystis psbE-psbF mutant lacking photosystem II required the presence of glucose even when grown in the light. These observations indicate that glnA transcription is under the control of the redox state of the cell. Finally, nitrogen starvation provoked a delay in the decrease of glnA transcript in darkness, suggesting a connection between nitrogen and redox controls of glnA transcript levels.
引用
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页码:789 / 799
页数:11
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