NOVEL GENOTYPES OF HEPATITIS-C VIRUS IN THAILAND

被引：19

作者：

SUGIYAMA, K

KATO, N

NAKAZAWA, T

YONEMURA, Y

PHORNPHUTKUL, K

KUNAKORN, M

PETCHCLAI, B

SHIMOTOHNO, K

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,CHUO KU,TOKYO 104,JAPAN

[2] KANAZAWA UNIV,SCH MED,DEPT SURG 2,KANAZAWA,ISHIKAWA 920,JAPAN

[3] CHAING MAI UNIV,FAC MED,CHIANG MAI 5000,THAILAND

[4] RAMATHIBODI HOSP,DEPT PATHOL,BANGKOK 10400,THAILAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-9-2323

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.

引用

收藏

页码：2323 / 2327

页数：5

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