PHORBOL ESTER-INDUCED SECRETION OF HUMAN HEPATOCYTE GROWTH-FACTOR BY HUMAN SKIN FIBROBLASTS AND ITS INHIBITION BY DEXAMETHASONE

被引：58

作者：

GOHDA, E

KATAOKA, H

TSUBOUCHI, H

DAIKILARA, Y

YAMAMOTO, I

机构：

[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT IMMUNOCHEM,OKAYAMA 700,JAPAN

[2] KAGOSHIMA UNIV,FAC MED,DEPT INTERNAL MED,KAGOSHIMA 890,JAPAN

[3] KAGOSHIMA UNIV,SCH DENT,DEPT BIOCHEM,KAGOSHIMA 890,JAPAN

来源：

FEBS LETTERS | 1992年 / 301卷 / 01期

关键词：

HUMAN HEPATOCYTE GROWTH FACTOR; PHORBOL ESTER; PROTEIN KINASE-C; HUMAN SKIN FIBROBLAST; DEXAMETHASONE; DNA SYNTHESIS;

D O I：

10.1016/0014-5793(92)80220-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human skin fibroblasts secreted a certain amount of human hepatocyte growth factor (hHGF), as determined by an enzyme-linked immunosorbent assay for hHGF. This hHGF secretion was remarkably stimulated by protein kinase C (PKC)-activating phorbol esters, which was inhibited by the simultaneous addition of dexamethasone. Pretreatment with phorbol 12-myristate 13-acetate (PMA) caused a down-regulation in hHGF secretion. hHGF secreted by the PMA-treated cells showed a potent hepatocyte growth-promoting activity which was neutralized by an anti-hHGF antiserum. These results indicate both that PMA-treated human skin fibroblasts produce biologically active hHGF and the possible involvement of PKC activation in this process.

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页码：107 / 110

页数：4

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