DIRECT SELECTION OF CDNAS USING WHOLE CHROMOSOMES

被引：12

作者：

ROUQUIER, S

TRASK, BJ

TAVIAUX, S

VANDENENGH, G

DIRIONG, S

LENNON, GG

GIORGI, D

机构：

[1] CTR RECH BIOCHIM MACROMOLEC,CNRS,UPR 9008,F-34033 MONTPELLIER,FRANCE

[2] UNIV WASHINGTON,DEPT MOLEC BIOTECHNOL FJ20,SEATTLE,WA 98195

[3] LAWRENCE LIVERMORE NATL LAB,LIVERMORE,CA 94551

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 21期

关键词：

D O I：

10.1093/nar/23.21.4415

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) and used to capture ds cDNAs by an improved magnetic bead capture protocol. To demonstrate the capabilities of this method, the selected cDNAs were used as probes in FISH experiments. The selected cDNA populations specifically painted chromosomes 17 or 19 on metaphase spreads. These results demonstrate that it is possible to do chromosome painting using cDNA probes and that this method is a means to rapidly select expressed sequences encoded by any portion of the genome.

引用

收藏

页码：4415 / 4420

页数：6

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