CRYSTALLIZATION OF THE SERYL-TRANSFER RNA SYNTHETASE-TRANSFER RNA(SER) COMPLEX OF ESCHERICHIA-COLI

被引：40

作者：

PRICE, S ^{[1
]}

CUSACK, S ^{[1
]}

BOREL, F ^{[1
]}

BERTHETCOLOMINAS, C ^{[1
]}

LEBERMAN, R ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,GRENOBLE OUTSTN,BP 156X,F-38042 GRENOBLE,FRANCE

来源：

FEBS LETTERS | 1993年 / 324卷 / 02期

关键词：

CRYSTALLIZATION; SERINE; AMINOACYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA; X-RAY STRUCTURE;

D O I：

10.1016/0014-5793(93)81386-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of the complex between seryl-tRNA synthetase and tRNA2ser from Escherichia coli have been obtained from ammonium sulphate solutions. The crystals are of the 1:2 enzyme:tRNA complex, belong to the space group C222(1), have cell dimensions of a = 128.9 angstrom, b = 164.9 angstrom, c = 127.3 angstrom and diffract anisotropically from 3.5 to 4.5 angstrom. An X-ray diffraction data set to 4 A has been collected. The combination of molecular replacement using the refined structure of the catalytic domain of the native enzyme, data from a heavy atom derivative and solvent flattening was used to produce a map at 4 angstrom resolution. This shows that a tRNA molecule binds across the dimer, the anticodon stem and loop do not contact the protein and the helical arm of the enzyme contacts the TPSIC loop and the long extra arm of the tRNA.

引用

页码：167 / 170

页数：4

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[No title captured]

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