Distribution of two molecular forms of gonadotropin-releasing hormone (GnRH) in the central nervous system of the frog Rana ridibunda

被引:43
作者
Collin, F
Chartrel, N
Fasolo, A
Conlon, JM
Vandesande, F
Vaudry, H
机构
[1] UNIV ROUEN, UA CNRS,INSERM,U413, CELLULAR & MOLEC NEUROENDOCRINOL LAB, F-76821 MONT ST AIGNAN, FRANCE
[2] UNIV TURIN, DEPT ANIM BIOL, I-10123 TURIN, ITALY
[3] CREIGHTON UNIV, SCH MED, DEPT BIOMED SCI, CTR REGULATORY PEPTIDE, OMAHA, NE 68178 USA
[4] CATHOLIC UNIV LEUVEN, INST ZOOL, NEUROENDOCRINOL LAB, B-3000 LOUVAIN, BELGIUM
关键词
neuropeptide; hypophysiotropic hormone; brain; amphibian; reproduction; immunohistochemistry;
D O I
10.1016/0006-8993(95)01074-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Two molecular forms of gonadotropin-releasing hormone (GnRH) have been recently characterized in the brain of the frog Rana ridibunda i.e. mammalian GnRH (mGnRH) and chicken GnRH-II (cGnRH-II). Using highly specific antisera against each form of GnRH, we have investigated the distribution of these two neuropeptides in the frog brain by the indirect immunofluorescence and the peroxidase-antiperoxidase techniques. mGnRH-immunoreactive cell bodies were restricted to a well defined region corresponding to the septal-anterior preoptic area, mGnRH-containing fibers projected through the ventral diencephalon and ended in the median eminence. In contrast, cGnRH-II-immunoreactive structures were widely distributed in the frog brain. In the telencephalon cGnRH-II-positive elements formed a ventromedial column extending from the olfactory bull, to the septal area, a pathway which corresponds to the terminal nerve. A dense accumulation of cGnRH-II-immunoreactive cell bodies was also found in the septal-anterior preoptic area; these neurons sent processes towards the median eminence via the hypothalamus. Double immunostaining revealed that, in this area, mGnRH- and cGnRH-II-like immunoreactivity co-existed in the same neurons. In the mid-diencephalon, numerous cGnRH-II-immunoreactive perikarya were found, surrounding the third ventricle, in the posterior preoptic and infundibular areas. Many of these neurons sent processes towards the ventricular cavity. More caudally, a dense population of cGnRH-II-immunoreactive perikarya was also observed in the nucleus of the paraventricular organ and the posterior tubercle. Dorsally, the thalamus, the tegmentum, the tectum and the granular layer of the cerebellum were richly innervated by cGnRH-II-positive fibers. In the medulla oblongata, numerous cGnRH-II-immunoreactive perikarya were seen in several cranial nerve nuclei. Ventrally, a dense plexus of immunoreactive fibers projected rostrocaudally into the spinal cord. The occurrence of mGnRH- and cGnRH-II-like immunoreactivity in the septal-anterior preoptic area and the hypothalamo-pituitary pathway supports the view that both peptides act as hypophysiotropic neurohormones. The widespread distribution of cGnRH-II-immunoreactive elements in the central nervous system of the frog strongly suggests that this peptide may also exert neuromodulator and/or neurotransmitter activities.
引用
收藏
页码:111 / 128
页数:18
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