AN IMPROVED HPLC METHOD FOR THE SEPARATION OF 14 CAROTENOIDS, INCLUDING 15-13-CIS-BETA-CAROTENE AND 9-CIS-BETA-CAROTENE ISOMERS, PHYTOENE AND PHYTOFLUENE

被引:21
作者
GRANADO, F
OLMEDILLA, B
BLANCO, I
ROJASHIDALGO, E
机构
[1] Nutrition Service, Clinica Puerta de Hierro, 28035, Madrid, c/ San Martin de Porres
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 13期
关键词
D O I
10.1080/01483919108049333
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, alpha-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, beta-apo-8' carotenal, beta-cryptoxanthin, echinenone, lycopene, gamma-carotene, alpha-carotene, beta-carotene, 9-cis-beta-carotene, 15-cis-/13-cis-beta-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15). The separation of the cis-isomers of beta-carotene is maintained, in spite of changes in the proportions and even after 250 hours of analysis. This chromatographic separation is not dependent on temperature (within a range of 15-37-degrees-C), on the injection solvent (THF, EtOH or the mobile phase) or flow rate (in a range of 1.3-2. 5 ml/min). the absorptivity value was unknown. The concentrations of the stock solutions were recalibrated monthly.
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页码:2457 / 2475
页数:19
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