CAPILLARY ELECTROPHORESIS ELECTROSPRAY-IONIZATION ION-TRAP MASS-SPECTROMETRY USING A SHEATHLESS INTERFACE

被引：39

作者：

RAMSEY, RS

MCLUCKEY, SA

机构：

[1] Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1995年 / 7卷 / 05期

关键词：

MASS SPECTROMETRY; ONLINE CAPILLARY ELECTROPHORESIS; MS/MS ANALYSIS; SHEATHLESS ELECTROSPRAY SOURCE; PEPTIDES;

D O I：

10.1002/mcs.1220070505

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The sensitivity that can be obtained with the quadrupole ion trap for on-line CE analysis using a sheathless electrospray interface and gold-tipped columns is examined. Capillaries of different i.d. are compared in continuous infusion experiments and the effects of sheath solvents on the signal for some peptides and proteins are illustrated. The sheathless CE/ITMS system using 20 mu m i.d. columns is shown to provide mid-attomole detection levels for leucine enkephalin in total ion electropherograms under full scan conditions. Good quality MS/MS spectra are also obtained for low femtomole injections of this peptide. (C) 1995 John Wiley & Sons, Inc.

引用

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页码：461 / 469

页数：9

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