大豆贮藏蛋白主要成分是7S和11S球蛋白,大豆贮藏蛋白组分及其亚基组成决定了蛋白质的品质和加工特性。本研究选用134对细胞核SSR标记,对166份栽培大豆微核心种质进行基因分型,运用一般线性回归(general linear model,GLM)和复合线性回归(mixed linear model,MLM)方法进行标记与性状的关联分析,定位大豆蛋白亚基的相关基因。结果表明,2年均检测到的且与蛋白亚基相关联的SSR位点有14个,以MLM方法检测到5个SSR位点(Sat062、Satt583、Satt291、Satt234和Satt595)与蛋白亚基相关联;7S组分各亚基变异程度较大,是引起11S/7S变异的主要原因;表型变异较大的亚基可能因为相关基因进化中发生重组较多,LD衰减距离较小,导致检测到较少的相关位点。本研究结果对蛋白亚基相关性状的标记辅助选择育种有重要的利用价值。
机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Jun, Tae-Hwan
Van, Kyujung
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机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Van, Kyujung
Kim, Moon Young
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Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South KoreaSeoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Kim, Moon Young
Lee, Suk-Ha
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机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Lee, Suk-Ha
Walker, David R.
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机构:
ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, USDA, Urbana, IL 61801 USASeoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
[10]
Linkage disequilibrium and association studies in higher plants: Present status and future prospects[J] . Pushpendra K. Gupta,Sachin Rustgi,Pawan L. Kulwal. Plant Molecular Biology . 2005 (4)
机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Jun, Tae-Hwan
Van, Kyujung
论文数: 0引用数: 0
h-index: 0
机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Van, Kyujung
Kim, Moon Young
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South KoreaSeoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Kim, Moon Young
Lee, Suk-Ha
论文数: 0引用数: 0
h-index: 0
机构:Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
Lee, Suk-Ha
Walker, David R.
论文数: 0引用数: 0
h-index: 0
机构:
ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, USDA, Urbana, IL 61801 USASeoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea
[10]
Linkage disequilibrium and association studies in higher plants: Present status and future prospects[J] . Pushpendra K. Gupta,Sachin Rustgi,Pawan L. Kulwal. Plant Molecular Biology . 2005 (4)