Study on Extraction Technological Conditions of Total Flavonoids from Toona sinensis Leaves

[Objective] The aim was to study the extraction technological conditions of total flavonoids from Toona sinensis leaves in order to provide theoretical basis for further development of T.sinensis leaves in the field of food and medicine.[Method] Spectrophotometry was adopted to determine the total flavonoids in T.sinensis leaves.By using single-factor test,the effects of ethanol concentration,solid-liquid ratio,temperature and time on extraction rate of the total flavonoids were analyzed.Based on this,the extraction technological conditions of the total foavonoids from T.sinensis leaves were optimized through orthogonal design method.[Result] The results showed that the optimal extracting conditions were as follows:ethanol concentration 70%,solid-liquid ratio 1:50,extracting duration 2.0 h and refluxing temperature 70℃.Under these optimum experimental conditions,the total flavonoids yield from T.sinensis leaves could reach 5.84%.[Conclusion] The test was repeated for 5 times under the optimum extraction conditions,and the mean yield of total flavonoids was 5.84%.Therefore,the method is stable and reliable.