Molecular identification, cloning and characterization of transmitted/founder HIV-1 subtype A, D and A/D infectious molecular clones

被引：57

作者：

Baalwa, Joshua ^{[1
]}

Wang, Shuyi ^{[3
]}

Parrish, Nicholas F. ^{[3
]}

Decker, Julie M. ^{[4
]}

Keele, Brandon F. ^{[5
]}

Learn, Gerald H. ^{[3
]}

Yue, Ling ^{[6
]}

Ruzagira, Eugene ^{[7
]}

Ssemwanga, Deogratius ^{[7
]}

Kamali, Anatoli ^{[7
]}

Amornkul, Pauli N. ^{[8
]}

Price, Matt A. ^{[8
]}

Kappes, John C. ^{[4
]}

Karita, Etienne ^{[6
]}

Kaleebu, Pontiano ^{[7
]}

Sanders, Eduard ^{[8
]}

Gilmour, Jill ^{[8
]}

Allen, Susan ^{[9
]}

Hunter, Eric ^{[6
]}

Montefiori, David C. ^{[10
]}

Haynes, Barton F. ^{[10
]}

Cormier, Emmanuel ^{[8
]}

Hahn, Beatrice H. ^{[3
]}

Shaw, George M. ^{[2
,3
]}

机构：

[1] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA

[2] Univ Penn, Perelman Sch Med, Dept Med, Philadelphia, PA 19104 USA

[3] Univ Penn, Dept Microbiol, Philadelphia, PA 19104 USA

[4] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA

[5] SAIC Frederick Inc, AIDS & Canc Virus Program, Frederick Natl Lab Canc Res, Ft Detrick, MD 21702 USA

[6] Emory Univ, Dept Pathol, Atlanta, GA 30322 USA

[7] Uganda Virus Res Inst, Med Res Council Program AIDS, Entebbe, Uganda

[8] Int AIDS Vaccine Initiat, New York, NY 10004 USA

[9] Emory Univ, Dept Global Hlth, Atlanta, GA 30322 USA

[10] Duke Univ, Sch Med, Duke Human Vaccine Inst, Durham, NC 27710 USA

来源：

VIROLOGY | 2013年 / 436卷 / 01期

关键词：

HIV-1; Transmitted/founder virus; Single genome sequencing; transmission; HIV-1 subtype A; HIV-1 subtype D; Neutralizing antibodies; HUMAN-IMMUNODEFICIENCY-VIRUS; FASTER DISEASE PROGRESSION; GP41 CYTOPLASMIC TAIL; 2ND CODING EXON; TYPE-1; SUBTYPE; HETEROSEXUAL TRANSMISSION; NEUTRALIZING ANTIBODIES; GENOTYPIC CHARACTERISTICS; ENVELOPE GLYCOPROTEINS; CORECEPTOR TROPISM;

D O I：

10.1016/j.virol.2012.10.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the molecular identification, cloning and initial biological characterization of 12 full-length HIV-1 subtype A, D and AID recombinant transmitted/founder (T/F) genomes. T/F genomes contained intact canonical open reading frames and all TIP viruses were replication competent in primary human T-cells, although subtype D virus replication was more efficient (p <0.05). All 12 viruses utilized CCR5 but not CXCR4 as a co-receptor for entry and exhibited a neutralization profile typical of tier 2 primary virus strains, with significant differences observed between subtype A and D viruses with respect to sensitivity to monoclonal antibodies VRC01, PG9 and PG16 and polyclonal subtype C anti-HIV IgG (p <0.05 for each). The present report doubles the number of T/F HIV-1 clones available for pathogenesis and vaccine research and extends their representation to include subtypes A, B, C and D. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：33 / 48

页数：16

共 73 条

[1] Novel envelope determinants for CCR3 use by human immunodeficiency virus [J].

Aasa-Chapman, Marlen M. I. ;

Seymour, Craig R. ;

Williams, Ian ;

McKnight, Aine .

JOURNAL OF VIROLOGY, 2006, 80 (21) :10884-10889

[2] Quantitating the Multiplicity of Infection with Human Immunodeficiency Virus Type 1 Subtype C Reveals a Non-Poisson Distribution of Transmitted Variants [J].

Abrahams, M. -R. ;

Anderson, J. A. ;

Giorgi, E. E. ;

Seoighe, C. ;

Mlisana, K. ;

Ping, L. -H. ;

Athreya, G. S. ;

Treurnicht, F. K. ;

Keele, B. F. ;

Wood, N. ;

Salazar-Gonzalez, J. F. ;

Bhattacharya, T. ;

Chu, H. ;

Hoffman, I. ;

Galvin, S. ;

Mapanje, C. ;

Kazembe, P. ;

Thebus, R. ;

Fiscus, S. ;

Hide, W. ;

Cohen, M. S. ;

Karim, S. Abdool ;

Haynes, B. F. ;

Shaw, G. M. ;

Hahn, B. H. ;

Korber, B. T. ;

Swanstrom, R. ;

Williamson, C. .

JOURNAL OF VIROLOGY, 2009, 83 (08) :3556-3567

[3] HIV-1 subtype D infection is associated with faster disease progression than subtype A in spite of similar plasma HIV-1 loads [J].

Baeten, Jared M. ;

Chohan, Bhavna ;

Lavreys, Ludo ;

Chohan, Vrasha ;

McClelland, R. Scott ;

Certain, Laura ;

Mandaliya, Kishorchandra ;

Jaoko, Walter ;

Overbaugh, Julie .

JOURNAL OF INFECTIOUS DISEASES, 2007, 195 (08) :1177-1180

[4]

Balasubramanian C., 2012, J BIOMOL STRUCT DYN, V29

[5] Early Low-Titer Neutralizing Antibodies Impede HIV-1 Replication and Select for Virus Escape [J].

Bar, Katharine J. ;

Tsao, Chun-yen ;

Iyer, Shilpa S. ;

Decker, Julie M. ;

Yang, Yongping ;

Bonsignori, Mattia ;

Chen, Xi ;

Hwang, Kwan-Ki ;

Montefiori, David C. ;

Liao, Hua-Xin ;

Hraber, Peter ;

Fischer, William ;

Li, Hui ;

Wang, Shuyi ;

Sterrett, Sarah ;

Keele, Brandon F. ;

Ganusov, Vitaly V. ;

Perelson, Alan S. ;

Korber, Bette T. ;

Georgiev, Ivelin ;

McLellan, Jason S. ;

Pavlicek, Jeffrey W. ;

Gao, Feng ;

Haynes, Barton F. ;

Hahn, Beatrice H. ;

Kwong, Peter D. ;

Shaw, George M. .

PLOS PATHOGENS, 2012, 8 (05)

[6] Wide Variation in the Multiplicity of HIV-1 Infection among Injection Drug Users [J].

Bar, Katharine J. ;

Li, Hui ;

Chamberland, Annie ;

Tremblay, Cecile ;

Routy, Jean Pierre ;

Grayson, Truman ;

Sun, Chuanxi ;

Wang, Shuyi ;

Learn, Gerald H. ;

Morgan, Charity J. ;

Schumacher, Joseph E. ;

Haynes, Barton F. ;

Keele, Brandon F. ;

Hahn, Beatrice H. ;

Shaw, George M. .

JOURNAL OF VIROLOGY, 2010, 84 (12) :6241-6247

[7] Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity [J].

Bhakta, Sushma J. ;

Shang, Liang ;

Prince, Jessica L. ;

Claiborne, Daniel T. ;

Hunter, Eric .

RETROVIROLOGY, 2011, 8

[8] HIV-1 subtype A envelope variants from early in infection have variable sensitivity to neutralization and to inhibitors of viral entry [J].

Blish, Catherine A. ;

Nedellec, Rebecca ;

Mandaliya, Kishorchandra ;

Mosier, Donald E. ;

Overbaugh, Julie .

AIDS, 2007, 21 (06) :693-702

[9] HIV-1 tropism and survival in vertically infected Ugandan infants [J].

Church, Jessica D. ;

Huang, Wei ;

Mwatha, Anthony ;

Toma, Jonathan ;

Stawiski, Eric ;

Donnell, Deborah ;

Guay, Laura A. ;

Mmiro, Francis ;

Musoke, Philippa ;

Jackson, J. Brooks ;

Parkin, Neil ;

Eshleman, Susan H. .

JOURNAL OF INFECTIOUS DISEASES, 2008, 197 (10) :1382-1388

[10] Evolution of CCR5 Use before and during Coreceptor Switching [J].

Coetzer, Mia ;

Nedellec, Rebecca ;

Salkowitz, Janelle ;

McLaughlin, Sherry ;

Liu, Yi ;

Heath, Laura ;

Mullins, James I. ;

Mosier, Donald E. .

JOURNAL OF VIROLOGY, 2008, 82 (23) :11758-11766

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