Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein

被引：178

作者：

Hecht, S

Eisenreich, W

Adam, P

Amslinger, S

Kis, K

Bacher, A

Arigoni, D

Rohdich, F

机构：

[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany

[2] ETH Honggerberg, Organ Chem Lab, CH-8093 Zurich, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 26期

关键词：

isoprenoid biosynthesis; 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate;

D O I：

10.1073/pnas.201399298

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recombinant Escherichia coli cells engineered for the expression of the xyIB gene in conjunction with genes of the nonmevalonate pathway were supplied with C-13-labeled 1-deoxy-D-xylulose. Cell extracts were analyzed directly by NMR spectroscopy. C-13-labeled 2C-methyl-D-erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xyIB, ispC, ispD, ispE, and ispF. The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway. Hypothetical mechanisms involving conserved cysteine residues are proposed for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate catalyzed by the GcpE protein.

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页码：14837 / 14842

页数：6

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