Photocleavable DNA Barcode-Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

被引：83

作者：

Agasti, Sarit S. ^{[1
]}

Liong, Monty ^{[1
]}

Peterson, Vanessa M. ^{[1
]}

Lee, Hakho ^{[1
]}

Weissleder, Ralph ^{[1
,2
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Syst Biol, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 45期

基金：

美国国家卫生研究院;

关键词：

TUMOR-CELLS; IMMUNO-PCR; STEM-CELLS; SENSORS;

D O I：

10.1021/ja307689w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout As a proof of principle, wedemonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.

引用

页码：18499 / 18502

页数：4

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