Fluorescent probe;
Hydrogen peroxide;
Naphthofluorescein;
Flow cytometry;
Near infrared;
D O I:
10.1016/j.bmcl.2008.08.035
中图分类号:
R914 [药物化学];
学科分类号:
100701 ;
摘要:
We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600 nm). Flow cytometry experiments in RAW264.7 macrophages establish that NPF1 can report changes in peroxide levels in living cells. (C) 2008 Elsevier Ltd. All rights reserved.
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Lee, Dongwon
;
Khaja, Sirajud
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Khaja, Sirajud
;
Velasquez-Castano, Juan C.
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机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Velasquez-Castano, Juan C.
;
Dasari, Madhuri
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机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Dasari, Madhuri
;
Sun, Carrie
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机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Sun, Carrie
;
Petros, John
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机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Petros, John
;
Taylor, W. Robert
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Taylor, W. Robert
;
Murthy, Niren
论文数: 0引用数: 0
h-index: 0
机构:
Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USAGeorgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Lee, Dongwon
;
Khaja, Sirajud
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Khaja, Sirajud
;
Velasquez-Castano, Juan C.
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Velasquez-Castano, Juan C.
;
Dasari, Madhuri
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Dasari, Madhuri
;
Sun, Carrie
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Sun, Carrie
;
Petros, John
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Petros, John
;
Taylor, W. Robert
论文数: 0引用数: 0
h-index: 0
机构:Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA
Taylor, W. Robert
;
Murthy, Niren
论文数: 0引用数: 0
h-index: 0
机构:
Georgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USAGeorgia Inst Technol, Wallace H Coulter Biomed Engn, Atlanta, GA 30332 USA