Comparative karyotyping as a tool for genome structure analysis of Trypanosoma cruzi

被引：18

作者：

Branche, C

Ochaya, S

Åslund, L

Andersson, B

机构：

[1] Karolinska Inst, Ctr Genom & Bioinformat, SE-17177 Stockholm, Sweden

[2] Rudbeck Lab, Dept Genet & Pathol, Uppsala, Sweden

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2006年 / 147卷 / 01期

关键词：

Trypanosoma cruzi; karyotype; genome; chromosome;

D O I：

10.1016/j.molbiopara.2006.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a part of the Trypanosoma cruzi genome project, 239 genetic markers were hybridised to PFGE separated DNA from 7: cruzi, in order to determine the number and size of chromosomes and to aid the assembly of the genome sequence. We used three strains, T. cruzi IIe CL Brener (the genome project reference strain) and two T. cruzi I strains, Sylvio X10/7 and CAI/72, to perform a comparative study of their karyotypes and to determine marker linkage. A densitometry analysis of the separations estimated the total chromosome numbers to be 55 in CL Brener and 57 in the two other strains. In all, 45 markers hybridised to single chromosomal bands and 103 markers to two bands in CL Brener, while the number of markers in Sylvio X 10/7 and CAI/72 were 102/68 and 61/105, respectively. Size differences between homologous chromosomes were often large, up to 1900 kb (173%). The average difference was 36% for CL Brener and 23.5% for the T. cruzi I strains. Larger differences in CL Brener are consistent with a recent hybrid origin. Forty markers distributed into 15 linkage groups were found to identify specific chromosomes or chromosomes pairs. While the same markers are generally linked in all three strains, the sizes of the chromosomes vary extensively, indicating large chromosomal rearrangements. These data provide valuable information for the finishing of the CL Brener genome sequence. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：30 / 38

页数：9

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