Application of anodized aluminum in fluorescence detection of biological species

被引：39

作者：

Takmakov, Pavel ^{[1
]}

Vlassiouk, Ivan ^{[1
]}

Smirnov, Sergei ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 385卷 / 05期

关键词：

biosensors; fluorescence/luminescence; nanoporous aluminum oxide; biotin;

D O I：

10.1007/s00216-006-0504-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thin nanoporous alumina obtained by anodization of aluminum films offers promising advantages for application in fluorescence-based biological sensors including convenient preparation, increased density of binding sites, and improved collection efficiency of fluorescence. These advantages are illustrated in the detection of streptavidin using biotin covalently bound to the surface of alumina nanopores. Fluorescence intensity enhancement as high as 7 times is observed in nanopores in comparison to flat glass surface.

引用

页码：954 / 958

页数：5

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