Hydrolyses and transglycosylations performed by purified α-D-glucosidase of the marine mollusc Aplysia fasciata

被引：26

作者：

Andreotti, G ^{[1
]}

Giordano, A ^{[1
]}

Trarnice, A ^{[1
]}

Mollo, E ^{[1
]}

Trincone, A ^{[1
]}

机构：

[1] CNR, Ist Chim Biomol, I-80078 Naples, Italy

来源：

JOURNAL OF BIOTECHNOLOGY | 2006年 / 122卷 / 02期

关键词：

alpha-glucosidase; transglycosylation; oligosaccharide synthesis; marine organism; Aplysia fasciata; biocatalysis;

D O I：

10.1016/j.jbiotec.2005.10.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The purification and characterisation of the alpha-glucosidase from the marine mollusc Aplysia fasciata are reported. Overall substrate specificity of the pure enzyme for both hydrolytic and transglycosylation reactions was studied. Remarkable characteristics of this enzyme are indicated by the results of the interesting survey of transglycosylation reactions reported: pyridoxine glucosylation, synthesis of chromophoric (pNP) di- and trisaccharides, glucosylation of cellobiose and sucrose. For these last two acceptors both the yields of reactions and the concentrations of products are comparable to those obtained using glycosyl transferases; in addition, synthesis of pyridoxine and chromophoric glycosides were still possible using a 1:1 ratio maltose:acceptor which is a very interesting characteristic from a synthetic point of view (effortless purification, productivity of each reaction batch, etc.). (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：274 / 284

页数：11

共 23 条

[1] Purification and characterization of a β-D-mannosidase from the marine anaspidean Aplysia fasciata [J].