Integrated microdevice for DNA restriction fragment analysis

被引：283

作者：

Jacobson, SC ^{[1
]}

Ramsey, JM ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB, DIV CHEM & ANALYT SCI, OAK RIDGE, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 05期

关键词：

D O I：

10.1021/ac951230c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An integrated monolithic device (8 mm x 10 mm) that performs an automated biochemical procedure is demonstrated. The device mixes a DNA sample with a restriction enzyme in a 0.7-nL reaction chamber and after a digestion period injects the fragments onto a 67-mm-long capillary electrophoresis channel for sizing. Materials are precisely manipulated under computer control within the channel structure using electrokinetic trans port. Digestion of the plasmid pBR322 by the enzyme HinfI and fragment analysis are completed in 5 min using 30 amol of DNA and 2.8 x 10(-3) unit of enzyme per run.

引用

页码：720 / 723

页数：4

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