Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

被引:88
作者
Gouveia-Caridade, Carla [1 ]
Pauliukaite, Rasa [1 ]
Brett, Christopher M. A. [1 ]
机构
[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal
关键词
multi-walled carbon nanotubes; carbon film electrodes; glucose oxidase; alcohol oxidase; eletrochemical biosensor;
D O I
10.1016/j.electacta.2008.01.040
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Functionalised multi-walled carbon nanotubes (MWCNTs) were cast oil glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified cat-boil film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:6732 / 6739
页数:8
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