Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

被引：88

作者：

Gouveia-Caridade, Carla ^{[1
]}

Pauliukaite, Rasa ^{[1
]}

Brett, Christopher M. A. ^{[1
]}

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

来源：

ELECTROCHIMICA ACTA | 2008年 / 53卷 / 23期

关键词：

multi-walled carbon nanotubes; carbon film electrodes; glucose oxidase; alcohol oxidase; eletrochemical biosensor;

D O I：

10.1016/j.electacta.2008.01.040

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Functionalised multi-walled carbon nanotubes (MWCNTs) were cast oil glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified cat-boil film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：6732 / 6739

页数：8

共 53 条

[21] Direct electrochemistry and bioelectrocatalysis of myoglobin at a carbon nanotube-modified electrode
Lue Ya-Fen
Yin Ya-Jing
Wu Ping
Cai Chen-Xin
[J]. ACTA PHYSICO-CHIMICA SINICA, 2007, 23 (01) : 5 - 11
[22] Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotube-modified electrodes
Luo, Xiliang
Killard, Anthony J.
Smyth, Malcolm R.
[J]. ELECTROANALYSIS, 2006, 18 (11) : 1131 - 1134
[23] VOLTAMMETRY IN THE PRESENCE OF ULTRASOUND - CAN ULTRASOUND MODIFY HETEROGENEOUS ELECTRON-TRANSFER KINETICS
MARKEN, F
EKLUND, JC
COMPTON, RG
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 395 (1-2): : 335 - 339
[24] Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes
Musameh, M
Wang, J
Merkoci, A
Lin, YH
[J]. ELECTROCHEMISTRY COMMUNICATIONS, 2002, 4 (10) : 743 - 746
[25] Fast electron transfer kinetics on multiwalled carbon nanotube microbundle electrodes
Nugent, JM
Santhanam, KSV
Rubio, A
Ajayan, PM
[J]. NANO LETTERS, 2001, 1 (02) : 87 - 91
[26] Reversible water-solubilization of single-walled carbon nanotubes by polymer wrapping
O'Connell, MJ
Boul, P
Ericson, LM
Huffman, C
Wang, YH
Haroz, E
Kuper, C
Tour, J
Ausman, KD
Smalley, RE
[J]. CHEMICAL PHYSICS LETTERS, 2001, 342 (3-4) : 265 - 271
[27] Fundamental electrochemical properties of carbon nanotube electrodes
Papakonstantinou, P
Kern, R
Robinson, L
Murphy, H
Irvine, J
McAdams, E
McLaughlin, J
McNally, T
[J]. FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES, 2005, 13 (02) : 91 - 108
[28] Screen-printed biosensors using different alcohol oxidases
Patel, NG
Meier, S
Cammann, K
Chemnitius, GC
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2001, 75 (1-2) : 101 - 110
[29] Long-range electrical contacting of redox enzymes by SWCNT connectors
Patolsky, F
Weizmann, Y
Willner, I
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (16) : 2113 - 2117
[30] Mediatorless catalytic oxidation of NADH at a disposable electrochemical sensor
Prasad, K. S.
Chen, J.-C.
Ay, C.
Zen, J.-M.
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2007, 123 (02): : 715 - 719

← 1 2 3 4 5 6 →