Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media

被引：90

作者：

Bedioui, F ^{[1
]}

Trevin, S ^{[1
]}

Devynck, J ^{[1
]}

Lantoine, F ^{[1
]}

Brunet, A ^{[1
]}

Devynck, MA ^{[1
]}

机构：

[1] UNIV PARIS 05,URA 1482 CNRS,LAB PHARMACOL CARDIOVASC,F-75015 PARIS,FRANCE

来源：

BIOSENSORS & BIOELECTRONICS | 1997年 / 12卷 / 03期

关键词：

nitric oxide; blood platelets; endothelial cells; electrochemical detection; porphyrinic sensor; ultramicroelectrodes;

D O I：

10.1016/S0956-5663(97)85338-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We describe here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein. (C) 1997 Elsevier Science Limited.

引用

页码：205 / 212

页数：8

共 39 条

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