Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups

被引：46

作者：

Bystrova, OV ^{[1
]}

Knirel, YA

Lindner, B

Kocharova, NA

Kondakova, AN

Zähringer, U

Pier, GB

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow, Russia

[2] Res Ctr Borstel, Ctr Med & Biosci, Borstel, Germany

[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Lab,Channing Lab, Boston, MA 02115 USA

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2006年 / 46卷 / 01期

关键词：

lipopolysaccharide structure; core oligosaccharide; O-unit; O-antigen; Pseudomonas aeruginosa;

D O I：

10.1111/j.1574-695X.2005.00004.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.

引用

页码：85 / 99

页数：15

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