A digital image-based flow-batch analyzer for determining Al(III) and Cr(VI) in water

This study proposes a digital image-based flow-batch analyzer (DIB-FBA) for aluminum (III) and chromium (VI) determinations in natural water. A webcam with a CCD sensor and RGB data are used. The method for determining aluminum is based on an Al(III) ion, quercetin, and cetyltrimethylammonium bromide (CTAB) reaction which yields a yellow colored complex in an acetate buffer medium (pH 5.5). The determination of chromium is based on a Cr(VI) ion with 1,5-diphenylcarbazide (DPC) reaction whose product is a violet colored complex. Digital images present the color of the emergent (complementary) radiation to that absorbed by the complexes formed in each analysis. RGB data were employed to build the analytical curves. All standard solutions were prepared in-line, and all analytical processes were completed by simply changing the operational parameters in the DIB-FBA control software. The working ranges were from 10 to 600 mu g L-1 for AI(III) with r=0.9994 (n=5), and 10 to 300 mu g L-1 for Cr(VI) with r=0.999 (n=5), the limits of detection were 3.97 and 2.65 mu g L-1 respectively for AI(III) and Cr(VI). The relative standard deviation (RSD %) and sampling rate were estimated at 1.5% and 137 h(-1) and at 1.7% and 134 h(-1) for Al (III) and Cr(VI), respectively. Comparing with the reference method, no statistically significant differences were observed when applying the paired t-test at a 95% confidence level. (C) 2012 Elsevier B.V. All rights reserved.