Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides

被引：85

作者：

Mukhopadhyay, Balaram ^{[1
,2
]}

Martins, Maristela B. ^{[3
]}

Karamanska, Rositsa ^{[3
]}

Russell, David A. ^{[1
]}

Field, Robert A. ^{[3
]}

机构：

[1] Univ E Anglia, Sch Chem Sci & Pharm, Norwich NR4 7TJ, Norfolk, England

[2] Indian Inst Sci Educ & Res Kolkata IISER K, Kolkata 700106, India

[3] John Innes Ctr, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England

来源：

TETRAHEDRON LETTERS | 2009年 / 50卷 / 08期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

STABILIZED GOLD NANOPARTICLES; COLORIMETRIC DETECTION; ESCHERICHIA-COLI; BIOLOGICAL DETECTION; LECTIN; BINDING; GLYCONANOPARTICLES; INFECTIONS; SURFACE; AGENTS;

D O I：

10.1016/j.tetlet.2008.12.029

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Mannose-coated CdS quantum dots (Man-QDs) were prepared in a facile aqueous, one-pot process that exploits the self-assembly of thiolated mannose in the presence of CdS under reducing conditions. The resulting similar to 15 nm diameter nanoparticles produce an intense, broad luminescence emission centred at 550 nm. These Man-QDs induce luminescent aggregates of Escherichia coli which can be used to detect bacteria in cell suspensions containing as few as 10(4) E. coli per mL. The aggregation process is dependent on the E coli cell surface FimH mannose-specific lectin. The recognition and subsequent detection of the E. coli using the Man-QD has been shown to be specific as aggregation does not occur either with an E. coli strain defective in the FimH lectin or with galactose-coated QDs. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：886 / 889

页数：4

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