Transient affinity tags based on the Dde protection/deprotection strategy: Synthesis and application of 2-biotinyl- and 2-hexanoyldimedone

被引：18

作者：

Kellam, B ^{[1
]}

Chan, WC ^{[1
]}

Chhabra, SR ^{[1
]}

Bycroft, BW ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,NOTTINGHAM NG7 2RD,ENGLAND

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 30期

关键词：

D O I：

10.1016/S0040-4039(97)01180-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

2-Biotinyl- and 2-hexanoyldimedone were prepared in a simple one step high yielding process by acylation of dimedone with biotin and hexanoic acid respectively, then without activation each attached to the N-terminus of resin-bound peptides. Following acidolytic side-chain deprotection and concomitant cleavage of the tagged peptides from the support, affinity purification was achieved on an avidin-agarose column and by RP-HPLC respectively. The purified peptides were finally released from the tag with 5% aqueous hydrazine. (C) 1997 Elsevier Science Ltd.

引用

页码：5391 / 5394

页数：4

共 12 条

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