Remote Optical Switch for Localized and Selective Control of Gene Interference

被引：170

作者：

Lee, Somin Eunice ^{[1
]}

Liu, Gang Logan ^{[2
]}

Kim, Franklin ^{[1
]}

Lee, Luke P. ^{[1
]}

机构：

[1] Univ Calif Berkeley, UCSF UCB Joint Grad Grp Bioengn, Dept Bioengn,Biomol Nanotechnol Ctr, Berkeley Sensor & Actuator Ctr, Berkeley, CA 94720 USA

[2] Univ Illinois, Micro & Nanotechnol Lab 3104, Dept Elect & Comp Engn, Urbana, IL 61801 USA

来源：

NANO LETTERS | 2009年 / 9卷 / 02期

基金：

美国国家卫生研究院;

关键词：

GOLD NANORODS; ENCAPSULATED MATERIALS; PHOTOTHERMAL THERAPY; LIVING CELLS; DNA; NANOPARTICLES; EXPRESSION; HYBRIDIZATION; ACTIVATION; AZOBENZENE;

D O I：

10.1021/nl802689k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Near infrared-absorbing gold nanoplasmonic particles (GNPs) are used as optical switches of gene interference and are remotely controlled using light. We have tuned optical switches to a wavelength where cellular photodamage is minimized. Optical switches are functionalized with double-stranded oligonucleotides. At desired times and at specific intracellular locations, remote optical excitation is used to liberate gene-interfering oligonucleotides. We demonstrate a novel gene-interfering technique offering spatial and temporal control, which is otherwise impossible using conventional gene-interfering techniques.

引用

页码：562 / 570

页数：9

共 43 条

[1] Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos [J].