Modified gold surfaces by 6-(ferrocenyl)hexanethiol/dendrimer/gold nanoparticles as a platform for the mediated biosensing applications

被引：38

作者：

Karadag, Murat ^{[1
]}

Geyik, Caner ^{[1
]}

Demirkol, Dilek Odaci ^{[1
]}

Ertas, F. Nil ^{[2
]}

Timur, Suna ^{[1
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey

[2] Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 02期

关键词：

Glucose biosensor; Gold nanoparticles; Self assembled monolayers; FLOW-INJECTION ANALYSIS; GLUCOSE-OXIDASE; ENZYME IMMOBILIZATION; COVALENT ATTACHMENT; CARBON NANOTUBES; DENDRIMER LAYERS; AU ELECTRODE; CONSTRUCTION; MONOLAYERS; MEMBRANE;

D O I：

10.1016/j.msec.2012.10.008

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

An electrochemical biosensor mediated by using 6-(Ferrocenyl) hexanethiol (FcSH) was fabricated by construction of gold nanoparticles (AuNPs) on the surface of polyamidoamine dendrimer (PAMAM) modified gold electrode. Glucose oxidase (GOx) was used as a model enzyme and was immobilized onto the gold surface forming a self assembled monolayer via FcSH and cysteamine. Cyclic voltammetry and amperometry were used for the characterization of electrochemical response towards glucose substrate. Following the optimization of medium pH, enzyme loading, AuNP and FcSH amount, the linear range for the glucose was studied and found as 1.0 to 5.0 mM with the detection limit (LOD) of 0.6 mM according to S/N = 3. Finally, the proposed Au/AuNP/(FcSH + Cyst)/PAMAM/GOx biosensor was successfully applied for the glucose analysis in beverages, and the results were compared with those obtained by HPLC. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：634 / 640

页数：7

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