Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications

被引：37

作者：

Emre, Fatma Bilge ^{[2
,3
]}

Ekiz, Fulya ^{[4
]}

Balan, Abidin ^{[2
,5
]}

Emre, Sinan ^{[6
]}

Timur, Suna ^{[1
]}

Toppare, Levent ^{[2
,4
,7
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey

[2] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[3] Inonu Univ, Fac Educ, TR-44280 Malatya, Turkey

[4] Middle E Tech Univ, Dept Biotechnol, TR-06531 Ankara, Turkey

[5] Eindhoven Univ Technol, Dept Chem Engn & Chem, Lab Macromol & Organ Chem, NL-5600 MB Eindhoven, Netherlands

[6] Celal Bayar Univ, Dept Ophthalmol, Fac Med, TR-45010 Manisa, Turkey

[7] Middle E Tech Univ, Dept Polymer Sci & Technol, TR-06531 Ankara, Turkey

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 158卷 / 01期

关键词：

Enzyme immobilization; Conducting polymer; Biosensor; Surface modification; AMPEROMETRIC GLUCOSE BIOSENSOR; PI-STACKING INTERACTIONS; 7-METHYLGUANINE BASE; NEUTRAL STATE; IMMOBILIZATION; OXIDASE; FILM;

D O I：

10.1016/j.snb.2011.05.052

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K-m and I-max) and surface morphologies. The biosensor was tested on real human blood serum samples. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：117 / 123

页数：7

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