Determination of free fatty acids by capillary zone electrophoresis

被引:24
作者
Buchberger, W
Winna, K
机构
[1] Department of Analytical Chemistry, Johannes-Kepler-University, A-4040 Linz
关键词
free fatty acids; dairy products; capillary electrophoresis;
D O I
10.1007/BF01252404
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Capillary zone electrophoresis was investigated for the separation of free fatty acids as an alternative to well established techniques such as GC or HPLC. The analysis was performed with indirect UV detection in a counter-electroosmotic flow mode using a diethylbarbiturate carrier electrolyte at a pH between 10 and 11 in a mixed aqueous-organic solvent. Separation of saturated and unsaturated fatty acids could be achieved after bromination of the double bonds. Problems with wall adsorption of fatty acids could be overcome by increasing the temperature and using a high concentration of a zwitterionic reagent to inactivate the silica surface. Increased sensitivity could be achieved after preconcentration on alumina. The method was applied to the determination of free fatty acids in dairy products. The advantages compared to traditional methods include short analysis times and simple preparation steps.
引用
收藏
页码:45 / 52
页数:8
相关论文
共 20 条
[11]   SEPARATION OF LONG AND SHORT CHAIN FATTY-ACIDS AS NAPHTHACYL AND SUBSTITUTED PHENACYL ESTERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
JORDI, HC .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1978, 1 (02) :215-230
[12]   CHEMILUMINESCENCE DETECTION OF FREE FATTY-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH IMMOBILIZED ENZYMES [J].
KAWASAKI, H ;
MAEDA, N ;
YUKI, H .
JOURNAL OF CHROMATOGRAPHY, 1990, 516 (02) :450-455
[13]   DETERMINATION OF FREE FATTY-ACIDS IN NATURAL OILS AND ALKYD RESINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
KING, JW ;
ADAMS, EC ;
BIDLINGMEYER, BA .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1982, 5 (02) :275-304
[14]   DIRECT, SENSITIVE AND SELECTIVE DETECTION OF FREE FATTY-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH POST-COLUMN ION-PAIR EXTRACTION AND ABSORBANCE DETECTION [J].
LAWRENCE, JF ;
CHARBONNEAU, CF .
JOURNAL OF CHROMATOGRAPHY, 1988, 445 (01) :189-197
[15]   GAS-CHROMATOGRAPHIC METHOD FOR DETERMINING FREE FATTY-ACIDS IN CHEESE [J].
MARTINHERNANDEZ, MC ;
ALONSO, L ;
JUAREZ, M ;
FONTECHA, J .
CHROMATOGRAPHIA, 1988, 25 (02) :87-90
[16]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SERUM LONG-CHAIN FATTY-ACIDS BY DIRECT DERIVATIZATION METHOD [J].
MIWA, H ;
YAMAMOTO, M ;
NISHIDA, T ;
NUNOI, K ;
KIKUCHI, M .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 416 (02) :237-245
[17]   OPTIMIZATION OF INORGANIC CAPILLARY ELECTROPHORESIS FOR THE ANALYSIS OF ANIONIC SOLUTES IN REAL SAMPLES [J].
ROMANO, J ;
JANDIK, P ;
JONES, WR ;
JACKSON, PE .
JOURNAL OF CHROMATOGRAPHY, 1991, 546 (1-2) :411-421
[18]   ELECTROPHORESIS IN FUSED-SILICA CAPILLARIES - THE INFLUENCE OF ORGANIC-SOLVENTS ON THE ELECTROOSMOTIC VELOCITY AND THE ZETA-POTENTIAL [J].
SCHWER, C ;
KENNDLER, E .
ANALYTICAL CHEMISTRY, 1991, 63 (17) :1801-1807
[19]   ANALYSIS OF UNDERIVATIZED C-12-C18 FATTY-ACIDS BY REVERSED-PHASE ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CONDUCTIVITY DETECTION [J].
TSUYAMA, Y ;
UCHIDA, T ;
GOTO, T .
JOURNAL OF CHROMATOGRAPHY, 1992, 596 (02) :181-184
[20]   LABELING OF FREE CARBOXYL GROUPS [J].
YASAKA, Y ;
TANAKA, M .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 659 (1-2) :139-155