A versatile strategy for electrochemical detection of hydrogen peroxide as well as related enzymes and substrates based on selective hydrogen peroxide-mediated boronate deprotection

We reported a versatile strategy for electrochemical determination of H(2)O(2) as well as related enzymes and substrates by combining the advantages of H(2)O(2)-mediated selective boronate deprotection and p-aminophenol for the first time. H(2)O(2) is selectively detected through the determination of p-aminophenol generated from H(2)O(2)-mediated boronate deprotection of p-aminophenylboronic acid. This method allows H(2)O(2) detection in the range from 1 mu M to 1 mM with a detection limit of 0.8 mu M. Since many enzymes and their substrates can be detected through the determination of H(2)O(2), we extend this method to detect related enzymes and their substrates using glucose and glucose oxidase as representative analytes. There is a good linear relationship between the current responses and GOx activity from 0.01 U/mL to 0.8 U/mL with a detection limit of 0.01 U/mL. The method is promising for broad and important applications, such as driver alcohol detection as well as clinical analysis of glucose, uric acid, lactate, glutamate, and so on. (C) 2011 Elsevier B.V. All rights reserved.