Wnt signalling regulates myogenic differentiation in the developing avian wing

被引:144
作者
Anakwe, K
Robson, L
Hadley, J
Buxton, P
Church, V
Allen, S
Hartmann, C
Harfe, B
Nohno, T
Brown, AMC
Evans, DJR
Francis-West, P [1 ]
机构
[1] Kings Coll London, Dept Craniofacial Dev, London SE1 9RT, England
[2] Barts & London, Queen Marys Sch Med & Dent, Dept Neurosci, London E1 4NS, England
[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[4] Kawasaki Med Sch, Dept Biol Mol, Kurashiki, Okayama 7010192, Japan
[5] Cornell Univ, Weill Med Coll, Dept Cell & Dev Biol, New York, NY 10021 USA
[6] Strang Canc Prevent Ctr, New York, NY 10021 USA
[7] Cardiff Univ, Sch Biosci, Cardiff CF10 3US, S Glam, Wales
来源
DEVELOPMENT | 2003年 / 130卷 / 15期
关键词
Wnt; limb; myogenic differentiation; fibre type; chick;
D O I
10.1242/dev.00538
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The limb musculature arises by delamination of premyogenic cells from the lateral dermomyotome. Initially the cells express Pax3 but, upon entering the limb bud, they switch on the expression of MyoD and Myf5 and undergo terminal differentiation into slow or fast fibres, which have distinct contractile properties that determine how a muscle will function. In the chick, the premyogenic cells express the Wnt antagonist Sfrp2, which is downregulated as the cells differentiate, suggesting that Wnts might regulate myogenic differentiation. Here, we have investigated the role of Wnt signalling during myogenic differentiation in the developing chick wing bud by gain- and loss-of-function studies in vitro and in vivo. We show that Wnt signalling changes the number of fast and/or slow fibres. For example, in vivo, Wnt11 decreases and increases the number of slow and fast fibres, respectively, whereas overexpression of Wnt5a or a dominant-negative Wnt11 protein have the opposite effect. The latter shows that endogenous Wnt11 signalling determines the number of fast and slow myocytes. The distinct effects of Wnt5a and Wnt11 are consistent with their different expression patterns, which correlate with the ultimate distribution of slow and fast fibres in the wing. Overexpression of activated calmodulin kinase 11 mimics the effect of Wnt5a, suggesting that it uses this pathway. Finally, we show that overexpression of the Wnt antagonist Sfrp2 and DeltaLef1 reduces the number of myocytes. In Sfrp2-infected limbs, the number of Pax3 expressing cells was increased, suggesting that Sfrp2 blocks myogenic differentiation. Therefore, Wnt signalling modulates both the number of terminally differentiated myogenic cells and the intricate slow/fast patterning of the limb musculature.
引用
收藏
页码:3503 / 3514
页数:12
相关论文
共 75 条
[41]   Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud [J].
Kengaku, M ;
Capdevila, J ;
Rodriguez-Esteban, C ;
De La Peña, J ;
Johnson, RL ;
Belmonte, JCI ;
Tabin, CJ .
SCIENCE, 1998, 280 (5367) :1274-1277
[42]  
Krüger M, 2001, DEVELOPMENT, V128, P743
[43]   The Wnt/Ca2+ pathway -: a new vertebrate Wnt signaling pathway takes shape [J].
Kühl, M ;
Sheldahl, LC ;
Park, M ;
Miller, JR ;
Moon, RT .
TRENDS IN GENETICS, 2000, 16 (07) :279-283
[44]   Ca2+/calmodulin-dependent protein kinase II is stimulated by Wnt and frizzled homologs and promotes ventral cell fates in Xenopus [J].
Kühl, M ;
Sheldahl, LC ;
Malbon, CC ;
Moon, RT .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (17) :12701-12711
[45]   Antagonistic regulation of convergent extension movements in Xenopus by Wnt/β-catenin and Wnt/Ca2+ signaling [J].
Kühl, M ;
Geis, K ;
Sheldahl, LC ;
Pukrop, T ;
Moon, RT ;
Wedlich, D .
MECHANISMS OF DEVELOPMENT, 2001, 106 (1-2) :61-76
[46]   Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development [J].
Ladher, RK ;
Church, VL ;
Allen, S ;
Robson, L ;
Abdelfattah, A ;
Brown, NA ;
Hattersley, G ;
Rosen, V ;
Luyten, FP ;
Dale, L ;
Francis-West, PH .
DEVELOPMENTAL BIOLOGY, 2000, 218 (02) :183-198
[47]   Identification of synergistic signals initiating inner ear development [J].
Ladher, RK ;
Anakwe, KU ;
Gurney, AL ;
Schoenwolf, GC ;
Francis-West, PH .
SCIENCE, 2000, 290 (5498) :1965-1967
[48]  
Lee CS, 2000, DEVELOPMENT, V127, P109
[49]   Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling [J].
Lewis, KE ;
Currie, PD ;
Roy, S ;
Schauerte, H ;
Haffter, P ;
Ingham, PW .
DEVELOPMENTAL BIOLOGY, 1999, 216 (02) :469-480
[50]   AN AVIAN MUSCLE FACTOR RELATED TO MYOD1 ACTIVATES MUSCLE-SPECIFIC PROMOTERS IN NONMUSCLE CELLS OF DIFFERENT GERM-LAYER ORIGIN AND IN BRDU-TREATED MYOBLASTS [J].
LIN, ZY ;
DECHESNE, CA ;
ELDRIDGE, J ;
PATERSON, BM .
GENES & DEVELOPMENT, 1989, 3 (07) :986-996