Synthesis and stability of novel terminal phosphate-labeled nucleotides

被引：7

作者：

Reynolds, Bambi ^{[1
]}

Miller, Rachel ^{[2
]}

Williams, John G. ^{[1
]}

Anderson, Jon P. ^{[1
]}

机构：

[1] LI COR Biosci Inc, Lincoln, NE 68504 USA

[2] Concordia Univ, Dept Athlet, Seward, NE USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2008年 / 27卷 / 01期

关键词：

PEG; phosphate-labeled nucleotide; single-molecule sequencing;

D O I：

10.1080/15257770701571768

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel compounds consisting of a nucleotide triphosphate labeled with a PEG linker and various terminal groups attached to the -phosphate of the nucleotide were constructed for use in efforts to produce a new class of DNA sequencer. The stability of these novel compounds was investigated to determine their utility as sequencing reagents. Hydrolysis rate constants were measured for both the natural nucleoside triphosphate dATP and novel dATP derivatives. The -labeled dATP was approximately 20-fold more stable to hydrolysis than dATP.

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收藏

页码：18 / 30

页数：13

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共 20 条

[1] COMPARISON OF METAL-ION-PROMOTED DEPHOSPHORYLATION OF 5'-TRIPHOSPHATES OF ADENOSINE, INOSINE, GUANOSINE AND CYTIDINE BY MN-2+, NI-2+ AND ZN-2+ IN BINARY AND TERNARY COMPLEXES [J].

AMSLER, PE ;

SIGEL, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 63 (02) :569-581

[2] ESCHERICHIA-COLI ENDONUCLEASE-III IS NOT AN ENDONUCLEASE BUT A BETA-ELIMINATION CATALYST [J].

BAILLY, V ;

VERLY, WG .

BIOCHEMICAL JOURNAL, 1987, 242 (02) :565-572

[3] Toward the $1000 human genome [J].

Bennett, ST ;

Barnes, C ;

Cox, A ;

Davies, L ;

Brown, C .

PHARMACOGENOMICS, 2005, 6 (04) :373-382

[4] RAPID DNA SEQUENCING BASED UPON SINGLE MOLECULE DETECTION [J].

DAVIS, LM ;

FAIRFIELD, FR ;

HARGER, CA ;

JETT, JH ;

KELLER, RA ;

HAHN, JH ;

KRAKOWSKI, LA ;

MARRONE, BL ;

MARTIN, JC ;

NUTTER, HL ;

RATLIFF, RL ;

SHERA, EB ;

SIMPSON, DJ ;

SOPER, SA .

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1991, 8 (01) :1-7

[5] Detecting single stranded DNA with a solid state nanopore [J].

Fologea, D ;

Gershow, M ;

Ledden, B ;

McNabb, DS ;

Golovchenko, JA ;

Li, JL .

NANO LETTERS, 2005, 5 (10) :1905-1909

[6] SINGLE-MOLECULE DETECTION AS AN APPROACH TO RAPID DNA SEQUENCING [J].

HARDING, JD ;

KELLER, RA .

TRENDS IN BIOTECHNOLOGY, 1992, 10 (1-2) :55-57

[7] Sequence-specific detection of individual DNA strands using engineered nanopores [J].

Howorka, S ;

Cheley, S ;

Bayley, H .

NATURE BIOTECHNOLOGY, 2001, 19 (07) :636-639

[8] Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis [J].

Kartalov, EP ;

Quake, SR .

NUCLEIC ACIDS RESEARCH, 2004, 32 (09) :2873-2879

[9] Terminal phosphate labeled nucleotides: Synthesis, applications, and linker effect on incorporation by DNA polymerases [J].

Kumar, S ;

Sood, A ;

Wegener, J ;

Finn, PJ ;

Nampalli, S ;

Nelson, JR ;

Sekher, A ;

Mitsis, P ;

Macklin, J ;

Fuller, CW .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2005, 24 (5-7) :401-408

[10] Fast DNA sequencing via transverse electronic transport [J].

Lagerqvist, J ;

Zwolak, M ;

Di Ventra, M .

NANO LETTERS, 2006, 6 (04) :779-782