A hybrid FIA/HPLC system incorporating monolithic column chromatography

被引：37

作者：

Adcock, Jacqui L.

Francis, Paul S. ^{[1
]}

Agg, Kent M.

Marshall, Graham D.

Barnett, Neil W.

机构：

[1] Deakin Univ, Sch Life & Environm Sci, Geelong, Vic 3217, Australia

[2] GlobalFIA, Fox Isl, WA 98333 USA

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 600卷 / 1-2期

基金：

澳大利亚研究理事会;

关键词：

flow injection analysis; gradient elution; monolithic column chromatography; chemiluminescence detection; neurotransmitter metabolites;

D O I：

10.1016/j.aca.2007.03.048

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5 -hydroxyindole - 3 -acetic acid and homovanillic acid) in human urine, using tris(2,2'-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：136 / 141

页数：6

共 28 条

[1] Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection [J].