LncRNA TUG1 is upregulated and promotes cell proliferation in osteosarcoma

被引:54
作者
Yun-Bo, Feng [1 ]
Xiao-Po, Liu [2 ]
Xiao-Li, Li [3 ]
Guo-Long, Cao [2 ]
Pei, Zhang [4 ]
Fa-Ming, Tian [5 ]
机构
[1] Tangshan Gongren Hosp, Tangshan 063000, Hebei Province, Peoples R China
[2] Tangshan Gongren Hosp, Dept Orthopaed, Tangshan 063000, Hebei Province, Peoples R China
[3] Tangshan City Workers Hosp, Dept Geriatr, Tangshan 063000, Hebei Province, Peoples R China
[4] Tangshan City Workers Hosp, Dept Neurol, Tangshan 063000, Hebei Province, Peoples R China
[5] North China Univ Sci & Technol, Ctr Med Expt Res, Tangshan 063000, Hebei Province, Peoples R China
关键词
TUG1; osteosarcoma; proliferation; AKT; LONG NONCODING RNAS; CANCER;
D O I
10.1515/med-2016-0031
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
Objective: To examine the expression and function of long non-coding RNA taurine up-regulated 1 (TUG1) in human osteosarcoma cells. Methods: Real-time quantitive PCR was used to detect the transcription level of TUG1 in a series of osteosarcoma cell lines. Knockdown of TUG1 in U2OS cells was carried out by transient transfection of siRNAs. MTT assay was performed to access the cell growth rates. Afterwards, RNA and protein of these cells were extracted to analyze the transfection efficient as well as the expression of other molecules. Results: Compared to the normal cell line, TUG1 exhibited a significant upregulation in osteosarcoma cells. Phenotyping analysis showed the growth-promotion activity of TUG1, since knockdown of TUG1 resulted in declined proliferation. We also found that AKT phosphorylation was impaired after TUG1 was inhibited, suggesting that the AKT pathway was involved in the regulation of TUG1 in U2OS cells. Conclusion: Our data provided evidence that TUG1 was upregulated and acted as a possible oncogene via positively regulating cell proliferation in osteosarcoma cells.
引用
收藏
页码:163 / 167
页数:5
相关论文
共 20 条
[1]
Osteosarcoma: ESMO Clinical Recommendations for diagnosis, treatment and follow-up [J].
Bielack, S. ;
Carrle, D. ;
Casali, P. G. .
ANNALS OF ONCOLOGY, 2009, 20 :137-139
[2]
Metastasis: recent discoveries and novel treatment strategies [J].
Eccles, Suzanne A. ;
Welch, Danny R. .
LANCET, 2007, 369 (9574) :1742-1757
[3]
Fatima Roshan, 2015, Mol Cell Ther, V3, P5
[4]
HOTAIR: an oncogenic long non-coding RNA in different cancers [J].
Hajjari, Mohammadreza ;
Salavaty, Abbas .
CANCER BIOLOGY & MEDICINE, 2015, 12 (01) :1-9
[5]
Long noncoding RNAs: Novel players in colorectal cancer [J].
Han, Dong ;
Wang, Meng ;
Ma, Ning ;
Xu, Ya ;
Jiang, Yuting ;
Gao, Xu .
CANCER LETTERS, 2015, 361 (01) :13-21
[6]
Long intergenic non-coding RNA TUG1 is overexpressed in urothelial carcinoma of the bladder [J].
Han, Yonghua ;
Liu, Yuchen ;
Gui, Yaoting ;
Cai, Zhiming .
JOURNAL OF SURGICAL ONCOLOGY, 2013, 107 (05) :555-559
[7]
Noncoding RNAs as potential biomarkers to predict the outcome in pancreatic cancer [J].
Jin, Kaizhou ;
Luo, Guopei ;
Xiao, Zhiwen ;
Liu, Zuqiang ;
Liu, Chen ;
Ji, Shunrong ;
Xu, Jin ;
Liu, Liang ;
Long, Jiang ;
Ni, Quanxing ;
Yu, Xianjun .
DRUG DESIGN DEVELOPMENT AND THERAPY, 2015, 9 :1247-1255
[8]
RNA maps reveal new RNA classes and a possible function for pervasive transcription [J].
Kapranov, Philipp ;
Cheng, Jill ;
Dike, Sujit ;
Nix, David A. ;
Duttagupta, Radharani ;
Willingham, Aarron T. ;
Stadler, Peter F. ;
Hertel, Jana ;
Hackermueller, Joerg ;
Hofacker, Ivo L. ;
Bell, Ian ;
Cheung, Evelyn ;
Drenkow, Jorg ;
Dumais, Erica ;
Patel, Sandeep ;
Helt, Gregg ;
Ganesh, Madhavan ;
Ghosh, Srinka ;
Piccolboni, Antonio ;
Sementchenko, Victor ;
Tammana, Hari ;
Gingeras, Thomas R. .
SCIENCE, 2007, 316 (5830) :1484-1488
[9]
Basic biology and therapeutic implications of lncRNA [J].
Khorkova, O. ;
Hsiao, J. ;
Wahlestedt, C. .
ADVANCED DRUG DELIVERY REVIEWS, 2015, 87 :15-24
[10]
Liu Yin, 2015, Front Biosci (Schol Ed), V7, P94