Sphingomonas sp. A1 lyase active on both poly-β-D-mannuronate and heteropolymeric regions in alginate

被引：30

作者：

Hashimoto, W ^{[1
]}

Okamoto, M

Hisano, T

Momma, K

Murata, K

机构：

[1] Kyoto Univ, Food Sci Res Inst, Uji 6110011, Japan

[2] Gunze Co Ltd, Kyoto Inst, Dept Biotechnol, Ayabe 6230051, Japan

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1998年 / 86卷 / 02期

关键词：

alginate; alginate lyase; poly-beta-D-mannuronate; Sphingomonas;

D O I：

10.1016/S0922-338X(98)80120-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alginate lyase III of Sphingomonas sp. A1 cleaved the glycosidic linkage of polymannuronate and heteropolymeric region composed of mannuronate and guluronate, but was inert on polyguluronate, The enzyme was observed to act endolytically, interact with tetrasaccharide in alginate, and form di- and trisaccharides as final products. This result suggests that the enzyme recognizes the unit of tetrasaccharide in alginate and cleaves the middle linkage of the tetrasaccharide.

引用

页码：236 / 238

页数：3

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