Preparing protein microarrays by soft-landing of mass-selected ions

被引：233

作者：

Ouyang, Z

Takáts, Z

Blake, TA

Gologan, B

Guymon, AJ

Wiseman, JM

Oliver, JC

Davisson, VJ

Cooks, RG ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

来源：

SCIENCE | 2003年 / 301卷 / 5638期

关键词：

D O I：

10.1126/science.1088776

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures and gently landed at different positions on a surface to form a microarray. An array of cytochrome c, lysozyme, insulin, and apomyoglobin was generated, and the deposited proteins showed electrospray ionization mass spectra that matched those of the authentic compounds. Deposited lysozyme and trypsin retained their biological activity. Multiply charged ions of protein kinase A catalytic subunit and hexokinase were also soft-landed into glycerol-based liquid surfaces. These soft-landed kinases phosphorylated LRRASLG oligopeptide and D-fructose, respectively.

引用

页码：1351 / 1354

页数：4

共 23 条

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