Involvement of miR169 in the nitrogen-starvation responses in Arabidopsis

被引：307

作者：

Zhao, Meng ^{[1
,2
,3
]}

Ding, Hong ^{[2
,3
]}

Zhu, Jian-Kang ^{[4
,5
]}

Zhang, Fusuo ^{[2
,3
]}

Li, Wen-Xue ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci Natl Key Facil Crop Genet Resources, Beijing 100081, Peoples R China

[2] China Agr Univ, Key Lab Plant & Soil Interact, Minist Educ, Beijing 100193, Peoples R China

[3] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China

[4] Univ Calif Riverside, Ctr Plant Cell Biol, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[5] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

来源：

NEW PHYTOLOGIST | 2011年 / 190卷 / 04期

基金：

美国国家科学基金会;

关键词：

Arabidopsis; miR169; NFYA; nitrate transporter; nitrogen starvation; AFFINITY NITRATE TRANSPORTER; TRANSCRIPTION FACTORS; CHL1; FUNCTIONS; MICRORNAS; PLANT; RNA; METABOLISM; NUTRIENT; STRESS; MIRNA;

D O I：

10.1111/j.1469-8137.2011.03647.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>Recent studies have revealed that microRNAs (miRNAs) regulate plant adaptive responses to nutrient deprivation. However, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. The Arabidopsis miR169 was strongly down-regulated, whereas its targets, NFYA (Nuclear Factor Y, subunit A) family members, were strongly induced by nitrogen N starvation. Analysis of the expression of miR169 precursors showed that MIR169a was substantially down-regulated in both roots and shoots by N starvation. Accumulation of the NFYA family members was suppressed in transgenic Arabidopsis with constitutive expression of MIR169a. Transgenic Arabidopsis plants overexpressing MIR169a accumulated less N and were more sensitive to N stress than the wild type. N sensitivity of 35S::MIR169a might be attributable to impaired uptake systems. These results provide evidence that miRNAs have functional roles in helping plants to cope with fluctuations in N availability in the soil.

引用

页码：906 / 915

页数：10

共 42 条

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