On-bead fluorescence assay for serine/threonine kinases

被引：26

作者：

Akita, S ^{[1
]}

Umezawa, N ^{[1
]}

Higuchi, T ^{[1
]}

机构：

[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi, Japan

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 25期

关键词：

D O I：

10.1021/ol052125k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHIC] A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.

引用

页码：5565 / 5568

页数：4

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