Identification of contaminating fungal DNA sequences in zymolyase

被引：28

作者：

Rimek, D

Garg, AP

Haas, WH

Kappe, R

机构：

[1] Univ Heidelberg, Childrens Hosp, Inst Hyg, D-6900 Heidelberg, Germany

[2] Univ Heidelberg, Childrens Hosp, Dept Gen Pediat, D-6900 Heidelberg, Germany

[3] C Charan Singh Univ, Dept Bot, Meerut, Uttar Pradesh, India

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 03期

关键词：

D O I：

10.1128/JCM.37.3.830-831.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls, The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase It appeared to he free from fungal DNA.

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页码：830 / 831

页数：2

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共 17 条

[1] RAPID DETECTION AND IDENTIFICATION OF CANDIDA-ALBICANS AND TORULOPSIS (CANDIDA) GLABRATA IN CLINICAL SPECIMENS BY SPECIES-SPECIFIC NESTED PCR AMPLIFICATION OF A CYTOCHROME-P-450 LANOSTEROL-ALPHA-DEMETHYLASE (L1A1) GENE FRAGMENT [J].

BURGENERKAIRUZ, P ;

ZUBER, JP ;

JAUNIN, P ;

BUCHMAN, TG ;

BILLE, J ;

ROSSIER, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (08) :1902-1907

[2] Detection and identification of fungal pathogens in blood by using molecular probes [J].

Einsele, H ;

Hebart, H ;

Roller, G ;

Loffler, J ;

Rothenhofer, I ;

Muller, CA ;

Bowden, RA ;

vanBurik, JA ;

Engelhard, D ;

Kanz, L ;

Schumacher, U .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) :1353-1360

[3] MICROTITRATION PLATE ENZYME-IMMUNOASSAY TO DETECT PCR-AMPLIFIED DNA FROM CANDIDA SPECIES IN BLOOD [J].

FUJITA, SI ;

LASKER, BA ;

LOTT, TJ ;

REISS, E ;

MORRISON, CJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) :962-967

[4] METHODS FOR DNA EXTRACTION FROM CANDIDA-ALBICANS [J].

GLEE, PM ;

RUSSELL, PJ ;

WELSCH, JA ;

PRATT, JC ;

CUTLER, JE .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :207-213

[5] Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa [J].

Haas, WH ;

Schilke, K ;

Brand, J ;

Amthor, B ;

Weyer, K ;

Fourie, PB ;

Bretzel, G ;

StichtGroh, V ;

Bremer, HJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (07) :1601-1603

[6] DETECTION OF CANDIDA-ALBICANS AND OTHER YEASTS IN BLOOD BY PCR [J].

HOLMES, AR ;

CANNON, RD ;

SHEPHERD, MG ;

JENKINSON, HF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (01) :228-231

[7] IDENTIFICATION AND ELIMINATION OF DNA-SEQUENCES IN TAQ DNA-POLYMERASE [J].

HUGHES, MS ;

BECK, LA ;

SKUCE, RA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (08) :2007-2008

[8] A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: Description of Saccharomyces kunashirensis sp nov and Saccharomyces martiniae sp nov [J].

James, SA ;

Chi, JP ;

Roberts, IN ;

Collins, MD .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (02) :453-460

[9] POLYMERASE CHAIN-REACTION FOR THE DIAGNOSIS OF CANDIDEMIA [J].

KAN, VL .

JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (03) :779-783

[10] Molecular probes for the detection of pathogenic fungi in the presence of human tissue [J].

Kappe, R ;

Okeke, CN ;

Fauser, C ;

Maiwald, M ;

Sonntag, HG .

JOURNAL OF MEDICAL MICROBIOLOGY, 1998, 47 (09) :811-820