Label-free optical detection and tracking of single virions bound to their receptors in supported membrane bilayers

被引：63

作者：

Ewers, Helge

Jacobsen, Volker

Klotzsch, Enrico

Smith, Alicia E.

Helenius, Ari

Sandoghdar, Vahid ^{[1
]}

机构：

[1] ETH, Phys Chem Lab, Nano Opt Grp, CH-8093 Zurich, Switzerland

[2] ETH, Inst Biochem, CH-8093 Zurich, Switzerland

来源：

NANO LETTERS | 2007年 / 7卷 / 08期

关键词：

D O I：

10.1021/nl070766y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We apply an interferometric optical detection scheme to image and track unlabeled single virions. Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound to supported membrane bilayers via the viral receptor, the glycolipid GM1. The technology presented here promises to be generally applicable to studying the motion of unlabeled macromolecules on membranes.

引用

收藏

页码：2263 / 2266

页数：4

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