An alternative bioreactor concept for application of an isolated oxidoreductase for asymmetric ketone reduction

In this paper an isolated NADH dependent ketone reductase has been used to synthesise (S)-6-bromo-beta-tetralol from 6-bromo-beta-tetralone, together with commercially available formate dehydrogenase (FDH) as a recycle enzyme to produce preparative quantities of the product. Furthermore, initial experiments indicate potential for an alternative bioreactor concept via the use of a resin (XAD L-323) to bind the product (and residual substrate) of the conversion rather than the cofactors or enzymes, thus allowing a new method of recycle, potentially overcoming existing problems. (C) 2003 Published by Elsevier Ltd.