Simultaneous electrochemical determination of epinephrine and uric acid at 1-butyl-4-methyl-pyridinium tetrafluroborate ionic liquid modified carbon paste electrode: A voltammetric study

An ionic liquid (IL) film 1-butyl-4-methyl-pyridinium tetrafluroborate modified carbon paste electrode (BMPTB/CPE) was fabricated and the electrochemical behavior of epinephrine at the modified electrode was investigated by cyclic voltammetric and differential pulse voltammetric techniques. A well-defined oxidation peak was observed at 0.21 V and the significant increase in peak current at modified carbon paste electrode compared to bare carbon paste electrode was obtained. The effect of scan rate on the oxidation of epinephrine was examined and it was found that the anodic peak current was proportional to the concentration of epinephrine in the range from 0.5 x 10(-5) to 4.5 x 10(-5) M. The BMPTB/CPE showed significantly higher sensitivity, and selectivity and used for the simultaneous determination of epinephrine and uric acid. The advantages of this biosensor showed simplicity, inherent stability and ability for simultaneous determination without using any other separation systems. The analytical performance of this biosensor has been evaluated for detection of epinephrine injection in serum as real sample. (C) 2011 Elsevier BM. All rights reserved.