Use of poly(tetrafluoroethylene)s as a sample support for the MALDI-TOF analysis of DNA and proteins

被引：71

作者：

Hung, KC ^{[1
]}

Ding, H ^{[1
]}

Guo, BC ^{[1
]}

机构：

[1] Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 02期

关键词：

D O I：

10.1021/ac980824n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization mass spectrometry of DNA and proteins, directly deposited on the poly(tetrafluoroethylene) (Teflon) surface, is demonstrated. For DNA analysis, this technique apparently produces a more homogeneous coverage of the matrix/DNA over the sample surface. Moreover, it enhances the sensitivity and salt tolerance. As described here, this technique can also achieve an excellent mass resolution, similar to that observed using a metal probe for DNA up to 62mer, We also examined the use of Tenon as a sample support for protein analysis since Tenon has been used as a transfer membrane. Less than 25 fmol of myoglobin has been detected with this technique. In addition, effective MALDI-TOF analysis of salt-contaminated protein samples can also be accomplished by loading the protein sample onto Tenon, followed by steps of washing away salts, adding the matrix, and desorbing sample directly from Tenon.

引用

页码：518 / 521

页数：4

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