Fluorescent chemodosimeter for Cys/Hcy with a large absorption shift and imaging in living cells

被引：123

作者：

Hu, Mingming ^{[1
]}

Fan, Jiangli ^{[1
]}

Li, Honglin ^{[1
]}

Song, Kedong ^{[2
]}

Wang, Song ^{[1
]}

Cheng, Guanghui ^{[1
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China

[2] Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116023, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2011年 / 9卷 / 04期

关键词：

INTRAMOLECULAR CHARGE-TRANSFER; COLORIMETRIC CHEMOSENSOR; PROBE; CYSTEINE; HOMOCYSTEINE; THIOLS; DESIGN; SENSOR; BOND;

D O I：

10.1039/c0ob00957a

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A novel molecule T1 with efficient intramolecular charge transfer was designed as a fluorescent chemodosimeter for cysteine (Cys) and homocysteine (Hcy). Upon addition of Cys/Hcy, T1 exhibited greatly enhanced fluorescence intensity as well as a large absorption peak shift (70 nm), and can be used for bioimaging of Cys/Hcy in living cells and detection in human plasma by visual color change. The detection mechanism was proved by H-1 NMR, mass spectrometry analysis and Gaussian calculations.

引用

页码：980 / 983

页数：4

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