On the extra-column band-broadening contributions of modern, very high pressure liquid chromatographs using 2 1 mm I D columns packed with sub-2 μm particles

被引:95
作者
Gritti, Fabrice [1 ]
Guiochon, Georges [1 ]
机构
[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA
基金
美国国家科学基金会;
关键词
Instrumentations; Band broadening; 1290 Infinity HPLC System; Acquity; Extra column effects; Efficiency; BEH C-18; PERFORMANCE; TEMPERATURE;
D O I
10.1016/j.chroma.2010.10.016
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The efficiencies of two narrow bore columns (100 mm and 50 mm x 2 1 mm) packed with 1 7 m totally porous BEH-C-18 particles were measured on two very high pressure liquid chromatographs (Acquity from Waters and 1290 Infinity H PLC System from Agilent) operating at maximum pressures of 1034 and 1200 bar respectively The probe compounds were a mixture of uracil acetophenone toluene and naphthalene eluted in a 50/50 (v/v) solution of acetonitrile and water at 303 K with a flow rate of 0 40 mL/min The apparent efficiencies of columns which lumps the consequences of band broadening due to the column and the system contributions may depend much on the extra-column volumes of the instruments used Actually it is known for a long time that the apparent column performance is strongly affected by the instrument characteristics including the diameter of the connecting tubes the injection technique (with or without needle seat capillary) and the detection cell volume When the 1290 Infinity HPLC System is equipped with a needle seat an Inlet and an outlet connecting capillary tube with inner diameters around 115 mu m its extra-column variance for a 0 1 mu l injection volume is 9 2 mu L-2 while that of the Acquity instrument is 6 9 mu L-2 Minor modifications suggested by their respective manufacturers allowed significant reductions of these variances to 6 2 and 3 9 mu(2) respectively Yet in their optimized configurations and for weakly retained compounds (k similar or equal to 1) these modern sophisticated instruments cannot provide more than 75% (1290 Infinity) and 85% (Acquity) of the maximum efficiency of a 2 1 mm x 50 mm BEH column For more strongly retained compounds (k>4) in contrast they are both able to provide more than 95% of the maximum expected efficiency (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码:7677 / 7689
页数:13
相关论文
共 18 条
[1]   ON THE DISPERSION OF A SOLUTE IN A FLUID FLOWING THROUGH A TUBE [J].
ARIS, R .
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1956, 235 (1200) :67-77
[2]   Characteristics of superficially-porous silica particles for fast HPLC:: Some performance comparisons with sub-2-μm particles [J].
DeStefano, J. J. ;
Langlois, T. J. ;
Kirkland, J. J. .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2008, 46 (03) :254-260
[3]   Effects of extra-column band spreading, liquid chromatography system operating pressure, and column temperature on the performance of sub-2-μm porous particles [J].
Fountain, Kenneth J. ;
Neue, Uwe D. ;
Grumbach, Eric S. ;
Diehl, Diane M. .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (32) :5979-5988
[4]   Influence of the pressure on the properties of chromatographic columns - I. Measurement of the compressibility of methanol-water mixtures on a mesoporous silica adsorbent [J].
Gritti, F ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1070 (1-2) :1-12
[5]   Heat exchanges in fast, high-performance liquid chromatography. A complete thermodynamic study [J].
Gritti, Fabrice ;
Guiochon, Georges .
ANALYTICAL CHEMISTRY, 2008, 80 (17) :6488-6499
[6]   Complete temperature profiles in ultra-high-pressure liquid chromatography columns [J].
Gritti, Fabrice ;
Guiochon, Georges .
ANALYTICAL CHEMISTRY, 2008, 80 (13) :5009-5020
[7]   Ultra high pressure liquid chromatography column permeability and changes of the eluent properties [J].
Gritti, Fabrice ;
Guiochon, Georges .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1187 (1-2) :165-179
[8]   Comparison between the efficiencies of columns packed with fully and partially porous C18-bonded silica materials [J].
Gritti, Fabrice ;
Cavazzini, Alberto ;
Marchetti, Nicola ;
Guiochon, Georges .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1157 (1-2) :289-303
[9]   Influence of the errors made in the measurement of the extra-column volume on the accuracies of estimates of the column efficiency and the mass transfer kinetics parameters [J].
Gritti, Fabrice ;
Felinger, Attila ;
Guiochon, Georges .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1136 (01) :57-72
[10]   Achieving the full performance of highly efficient columns by optimizing conventional benchmark high-performance liquid chromatography instruments [J].
Gritti, Fabrice ;
Sanchez, Carl A. ;
Farkas, Tivadar ;
Guiochon, Georges .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (18) :3000-3012