Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

被引：71

作者：

Zhang, Jiangyan ^{[1
]}

Li, Zhengping ^{[1
]}

Wang, Hui ^{[1
]}

Wang, Yucong ^{[1
]}

Jia, Hongxia ^{[1
]}

Yan, Jingli ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Minist Educ, Key Lab Med Chem & Mol Diag, Baoding 071002, Hebei Province, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 33期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

QUANTITATION;

D O I：

10.1039/c1cc13466c

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.

引用

收藏

页码：9465 / 9467

页数：3

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