Immobilization of beta-glucosidase from a commercial preparation .1. A comparative study of natural supports

被引:37
作者
Martino, A
Durante, M
Pifferi, PG
Spagna, G
Bianchi, G
机构
[1] Faculty of Industrial Chemistry, University of Bologna, 40136 Bologna
关键词
D O I
10.1016/0032-9592(95)00065-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A number of natural supports (cellulose PEI, alpha-alumina, gamma-alumina and chitosan) were tested for the immobilization of beta-glucosidase in view of its possible application in the wine-making and fruit-juice processing industry. Immobilization on chitosan produced an active catalyst (about 500 U/g) and a shift of pH optimum to lower values, which is of considerable interest for use in the beverage processing industry. However, the short storage life of the immobilized enzyme is currently a drawback to ifs industrial applications.
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页码:281 / 285
页数:5
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