Biosensor for total cholesterol estimation using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer

被引：22

作者：

Arya, Sunil K.

Datta, Monika

Singh, S. P.

Malhotra, Bansi D.

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, New Delhi 110007, India

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 7-8期

关键词：

cholesterol oleate; N-(2-Aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS); self-assembled monolayer (SAM); cyclic voltammetry; biosensor;

D O I：

10.1007/s00216-007-1655-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol oxidase (ChOx), cholesterol esterase (ChEt), and horseradish peroxidase (HRP) have been co-immobilized covalently on a self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS) deposited on an indium-tin-oxide (ITO) glass surface. These enzyme-modified (ChOx-ChEt-HRP/AEAPTS/ITO) biosensing electrodes have been used to estimate cholesteryl oleate from 10 to 500 mg dL(-1). The sensitivity, K-m value, and shelf-life of these ChEt-ChOx-HRP/AEAPTS/ITO biosensing electrodes have been found to be 124 nA mg(-1) dL, 95.098 mg dL(-1) (1.46 mmol L-1), and ten weeks, respectively. The ChEt-ChOx-HRP/AEAPTS/ITO bio-electrodes have been used to estimate total cholesterol in serum samples.

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收藏

页码：2235 / 2242

页数：8

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