Genome-wide application of RNAi to the discovery of potential drug targets

被引：31

作者：

Ito, M

Kawano, K

Miyagishi, M

Taira, K

机构：

[1] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562, Japan

[2] iGENE Therapeut Inc, Tsukuba, Ibaraki 3058562, Japan

[3] Univ Tokyo, Sch Med, 21st Century Ctr Excellence Program, Tokyo 1138655, Japan

[4] Univ Tokyo, Sch Engn, Dept Chem & Biochem, Tokyo 1138656, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 26期

关键词：

RNA interference; small interfering RNA; vector-based siRNA;

D O I：

10.1016/j.febslet.2005.08.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Progress is being made in the development of RNA interference-based (RNAi-based) strategies for the control of gene expression. It has been demonstrated that small interfering RNAs (siRNAs) can silence the expression of target genes in a sequence-specific manner in mammalian cells. Various groups, including our own, have developed systems for vector-mediated specific RNAi. Vector-based siRNA- (or shRNA) expression libraries directed against the entire human genome and siRNA libraries based on chemically synthesized oligonucleotides now allow the rapid identification of functional genes and potential drug targets. Use of such libraries will enhance our understanding of numerous biological phenomena and contribute to the rational design of drugs against heritable, infectious and malignant diseases. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5988 / 5995

页数：8

共 98 条

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Genome-wide RNAi screen for host factors required for intracellular bacterial infection [J].