An indirect ELISA and a PCR technique for the detection of Grouper (Epinephelus marginatus) mislabeling

被引：31

作者：

Asensio, Luis ^{[1
]}

Gonzalez, Isabel ^{[2
]}

Pavon, Miguel A. ^{[2
]}

Garcia, Teresa ^{[2
]}

Martin, Rosario ^{[2
]}

机构：

[1] Univ San Pablo CEU, Fac Farm, Dept Nutr Bromatol & Tecnol Alimentos, Madrid 28668, Spain

[2] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2008年 / 25卷 / 06期

关键词：

grouper; adulteration; multiplex PCR; ELISA;

D O I：

10.1080/02652030701765731

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) and a multiplex polymerase chain reaction (PCR) procedure was applied for the detection of Grouper (Epinephelus marginatus) mislabelling in the fish market. An indirect ELISA (microtiter-plate format) using two monoclonal antibodies (3D12 and 1A4) was assayed and multiplex PCR performed using species-specific primers of the 5S rDNA gene for the rapid authentication of grouper. A total of 70 commercial fish fillet samples, collected from local markets and supermarkets, labelled as grouper were analysed: 12 of the 70 samples were confirmed to be Grouper. The PCR technique permitted the detection of Nile Perch (Lates niloticus) in the commercial fillet samples, which was not possible using ELISA. The results suggest that both ELISA and PCR are specific and reliable tools for the detection of Grouper mislabelling/adulteration and the accurate implementation of traceability for successful regulatory food controls.

引用

页码：677 / 683

页数：7

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