Fabrication of a novel impedance cell sensor based on the polystyrene/polyaniline/Au nanocomposite

被引：45

作者：

Gu, Miaomiao ^{[1
]}

Zhang, Jingjing ^{[1
]}

Li, Ye ^{[1
]}

Jiang, Liping ^{[1
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China

来源：

TALANTA | 2009年 / 80卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Gold nanoparticles; PS@PANI; HL-60; cells; Biosensor; Electrochemical impedance spectroscopy; SELF-ASSEMBLED MONOLAYER; ESCHERICHIA-COLI O157-H7; GOLD NANOPARTICLES; CANCER-CELLS; IMMUNOSENSOR; ELECTRODES;

D O I：

10.1016/j.talanta.2009.06.065

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gold nanoparticles (AuNPs) were assembled on the surface of polystyrene (PS) and polyaniline (PANI) core-shell nanocomposite (PS@PANI) for the immobilization of HL-60 leukemia cells to fabricate a cell electrochemical sensor. The immobilized cells exhibited irreversible voltammetric response and increased the electron transfer resistance with a good correlation to the logarithmic value of concentration ranging from 1.6 x 10(3) to 1.6 x 10(8) cells mL(-1) with a limit of detection of 7.3 x 10(2) cells mL(-1) at 10 sigma. This biosensor was simple, low cost and disposable, which implied that the PS@PANI/Au composites can regard as the potential applications for clinical applications. (C) 2009 Elsevier B.V. All rights reserved.

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收藏

页码：246 / 249

页数：4

相关论文

共 14 条

[1] Photoelectric behavior of mammalian cells and its bioanalytical applications [J].

Ci, YX ;

Zhang, CY ;

Feng, J .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 45 (02) :247-251

[2] A self-assembled monolayer based electrochemical immunosensor for detection of leukemia K562A cells [J].

Hao, Chen ;

Yan, Feng ;

Ding, Lin ;

Xue, Yadong ;

Ju, Huangxian .

ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (06) :1359-1364

[3] Rapid identification and high sensitive detection of cancer cells on the gold nanoparticle interface by combined contact angle and electrochemical measurements [J].

He, Fang ;

Shen, Qin ;

Jiang, Hui ;

Zhou, Jian ;

Cheng, Jian ;

Guo, Dadong ;

Li, Qingning ;

Wang, Xuemei ;

Fu, Degang ;

Chen, Baoan .

TALANTA, 2009, 77 (03) :1009-1014

[4] An immunosensor for haemoglobin based on impedimetric properties of a new mixed self-assembled monolayer [J].

Hleli, S ;

Martelet, C ;

Abdelghani, A ;

Bessueille, F ;

Errachid, A ;

Samitier, J ;

Hays, HCW ;

Millner, PA ;

Bums, N ;

Jaffrezic-Renault, N .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2006, 26 (2-3) :322-327

[5] Detection and distinguishability of leukemia cancer cells based on Au nanoparticles modified electrodes [J].

Hu, Hong-Li ;

Jiang, Hui ;

Wang, Xue-Mei ;

Chen, Bao-An .

ELECTROCHEMISTRY COMMUNICATIONS, 2008, 10 (08) :1121-1124

[6] Impedance sensing of DNA binding drugs using gold substrates modified with gold nanoparticles [J].

Li, CZ ;

Liu, YL ;

Luong, JHT .

ANALYTICAL CHEMISTRY, 2005, 77 (02) :478-485

[7] Gold nanoparticles induce oxidative damage in lung fibroblasts in vitro [J].

Li, Jasmine J. ;

Zou, Li ;

Hartono, Deny ;

Ong, Choon-Nam ;

Bay, Boon-Huat ;

Yung, Lin-Yue Lanry .

ADVANCED MATERIALS, 2008, 20 (01) :138-+

[8] Fabrication of a novel glucose biosensor based on a highly electroactive polystyrene/polyaniline/Au nanocomposite [J].

Liu, Yuge ;

Feng, Xiaomiao ;

Shen, Jianmin ;

Zhu, Jun-Jie ;

Hou, Wenhua .

JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (30) :9237-9242

[9] Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles [J].

Morrin, A ;

Wilbeer, F ;

Ngamna, O ;

Moulton, SE ;

Killard, AJ ;

Wallace, GG ;

Smyth, MR .

ELECTROCHEMISTRY COMMUNICATIONS, 2005, 7 (03) :317-322

[10] Electrodiffusion in ionic channels of biological membranes [J].

Nonner, W ;

Eisenberg, B .

JOURNAL OF MOLECULAR LIQUIDS, 2000, 87 (2-3) :149-162